Isolation of a lipase-producing Trichosporon spp and enzyme extraction by two-phase aqueous system

Isolation of a lipase-producing Trichosporon spp and enzyme extraction by two-phase aqueous system

A lipase-producing yeast strain isolated from crude cheese and identified as Trichosporon spp produced 7.3 U/mL (59.3 U/µg) after 72h of cultivation. Lipase showed optimum activity at pH 7.0-8.0 and 45-50ºC. Extraction by the two-phase aqueous system (PEG-phosphate salts) showed an elevated recupera...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Brazilian journal of microbiology 2007-01, Vol.38 (1), p.62-64
Hauptverfasser: Santos, Juliana A.(University of Pernambuco Institute of Biological Sciences ,Federal University of Pernambuco Department of Genetics Laboratory of Imunopathology Keizo Asami), Amaral, Marcel C.O.(Federal University of Pernambuco Department of Genetics Laboratory of Imunopathology Keizo Asami), Araújo, Thiago A.S.(University of Pernambuco Institute of Biological Sciences ,University of Pernambuco Department of Exact and Natural Sciences), Fernandes, Késsia G.C.(University of Pernambuco Institute of Biological Sciences ,University of Pernambuco Department of Exact and Natural Sciences), Chaves, Adilson C.(University of Pernambuco Institute of Biological Sciences ,University of Pernambuco Department of Exact and Natural Sciences), Morais, Márcia M.C.(University of Pernambuco Institute of Biological Sciences)
Format: Artikel
Sprache:eng
Schlagworte:
aqueous two phase system
extracellular lipase
lipase extracelular
MICROBIOLOGY
PEG
sistema bifásico aquoso
Trichosporon
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:A lipase-producing yeast strain isolated from crude cheese and identified as Trichosporon spp produced 7.3 U/mL (59.3 U/µg) after 72h of cultivation. Lipase showed optimum activity at pH 7.0-8.0 and 45-50ºC. Extraction by the two-phase aqueous system (PEG-phosphate salts) showed an elevated recuperation (99.8%) of enzymatic activity in the PEG phase. Uma levedura produtora de lipase isolada de queijo coalho e identificada como Trichosporon spp produziu 7,3 U/mL (59,3 U/µg) após 72h de cultivo. A lipase mostrou atividade ótima em pH 7,0-8,0 e temperatura ótima entre 45-50ºC. Extração pelo sistema PEG - sais de fosfato apresentou 99,8% de recuperação da atividade enzimática na fase PEG.
ISSN:1517-8382
1678-4405
1678-4405
DOI:10.1590/S1517-83822007000100013