Genetic variability of brazilian Alternaria spp. isolates as revealed by RAPD analysis

The genetic variability of 22 isolates of the fungi Alternaria alternata and A. cassiae, obtained from Senna obtusifolia, was studied by RAPD analysis. A total of 491 scorable bands were produced with the use of 28 primers. Cluster analysis based on similarities computed from RAPD markers showed two...

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Veröffentlicht in:Brazilian journal of microbiology 2003-06, Vol.34 (2), p.117-119
Hauptverfasser: Tigano, Myrian Silvana(Empresa Brasileira de Pesquisa Agropecuária Recursos Genéticos e Biotecnologia), Aljanabi, Salah(Biology Department), Mello, Sueli Corrêa Marques de(Empresa Brasileira de Pesquisa Agropecuária Recursos Genéticos e Biotecnologia)
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Sprache:eng
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Zusammenfassung:The genetic variability of 22 isolates of the fungi Alternaria alternata and A. cassiae, obtained from Senna obtusifolia, was studied by RAPD analysis. A total of 491 scorable bands were produced with the use of 28 primers. Cluster analysis based on similarities computed from RAPD markers showed two distinct genetic groups of isolates related to both species. RAPD analysis proved to be an efficient method for detecting genetic variability of A. cassiae and A. alternata isolates occurring in S. obtusifolia, and also for distinguishing Alternaria species. A variabilidade genética entre 22 isolados dos fungos Alternaria alternata e A. Cassiae, obtidos de Senna obtusifolia, foi estudada através da análise de RAPD. No total, 491 bandas foram selecionadas com o uso de 28 primers. O agrupamento baseado em similaridades computadas dos marcadores mostrou dois distintos grupos de isolados relacionados às espécies. A análise de RAPD provou ser um método eficiente para detecção da variabilidade genética entre isolados de A. alternata e A. cassiae ocorrendo em S. obtusifolia, e também para distinguir as espécies de Alternaria.
ISSN:1517-8382
1678-4405
1678-4405
1517-8382
DOI:10.1590/S1517-83822003000200005