Sensitivity of rabbit fibrochondrocytes to mycoplasmas
Primary cell culture from rabbit meniscus (fibrochondrocytes-FcrC) was infected for 24 hours with different inocula (10² to 10(7) Colony Forming Units-CFU) of Mycoplasma hominis PG-21, M. pneumoniae FH and 1428 or M. arthritidis PG-6. The severity of the different obtained cytophatic effects-CPE was...
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Veröffentlicht in: | Brazilian journal of microbiology 2002-09, Vol.33 (3), p.243-246 |
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Sprache: | eng |
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Zusammenfassung: | Primary cell culture from rabbit meniscus (fibrochondrocytes-FcrC) was infected for 24 hours with different inocula (10² to 10(7) Colony Forming Units-CFU) of Mycoplasma hominis PG-21, M. pneumoniae FH and 1428 or M. arthritidis PG-6. The severity of the different obtained cytophatic effects-CPE was inoculum, Mycoplasma species and strain dependant. These bacteria were recovered from all infected FcrC and the SP4 medium for mycoplasmas also caused toxic effect on the FcrC. It was concluded that rabbit fibrochondrocytes were sensitive to mycoplasma infection, as well as to the SP4 mycoplasma medium.
Cultura primária de menisco de coelho (Fibrocondrócito-FcrC) foi infectada por 24 horas com diferentes inóculos (10² to 10(7) Unidade Formadoras de Colonias-UFC) de Mycoplasma hominis PG-21, M. pneumoniae FH e 1428 ou M. arthritidis PG-6. A severidade dos diferentes efeitos citopáticos-EC foram dependentes do inóculo, espécie e cepa de micoplasma. Estas bactérias foram reisoladas de todos os FcrC infectados e o meio SP4 para micoplasmas também causou efeito tóxico para os FcrC. Concluiu-se que os fibrocondrócitos de coelho foram sensíveis à infecção por micoplasmas e também ao meio SP4. |
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ISSN: | 1517-8382 1678-4405 1678-4405 |
DOI: | 10.1590/s1517-83822002000300011 |