Preliminary approach to detect amylolytic and pectinolytic activities from maca (Lepidium meyenii Walp.)

Amylase (AM), pectinesterase (PE) and polygalacturonase (PG) were extracted from maca roots (Lepidium meyenii Walp). The response surface model was used to determine the optimum conditions of pH and temperature activity of each enzyme on the crude extract. The highest values of AM activity occurred...

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Veröffentlicht in:Revista brasileira de ciências farmacêuticas 2006-03, Vol.42 (1), p.49-58
Hauptverfasser: Rondan-Sanabria, Gerby Giovanna, Pires, Tatiana da Costa Raposo, Finardi Filho, Flavio
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Sprache:eng
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Zusammenfassung:Amylase (AM), pectinesterase (PE) and polygalacturonase (PG) were extracted from maca roots (Lepidium meyenii Walp). The response surface model was used to determine the optimum conditions of pH and temperature activity of each enzyme on the crude extract. The highest values of AM activity occurred at pH 6.1 and 33.6 °C, very close to the experimental central point. The PE activity had a maximum activity at pH 6.6 and 49.4 and PG activity showed optimum activity at pH 5.4 and 46 °C.
ISSN:1516-9332
1516-9332
DOI:10.1590/S1516-93322006000100005