Replication kinetics of novel swine influenza A viruses: an approach to vaccine production

Novel swine Influenza A viruses (IAVs) have been described in South America. The objective of this study was to evaluate the replication kinetics of novel swine IAVs as a first step for vaccine production. Different swine IAV lineages (H1N1, H1N2, and H3N2), infection doses (MOI: 1, 0.1, 0.01, 0.001, 0.0001, and 0.00001), harvest times (every 12 h), and substrates (MDCK and Vero cells) were used. For all IAV strains, MDCK cells were the most efficient substrate, generating titers greater than or equal to 128 HAU/50 µL with an MOI of 0.00001 at 60 h post-infection. These data could be useful for vaccine-producing laboratories.