Bacterial Cellulose Production by Komagataeibacter hansenii ATCC 23769 Using Sisal Juice - An Agroindustry Waste

ABSTRACT In this study, we have optimized production of bacterial cellulose (BC) by Komagataeibacter hansenii ATCC 23769 in a static cultivation using sisal juice, an agroindustrial residue, as substrate. Optimization of fermentation parameters has been carried out using the one-variable-at-a-time m...

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Veröffentlicht in:Brazilian journal of chemical engineering 2017-07, Vol.34 (3), p.671-680
Hauptverfasser: Lima, H. L. S., Nascimento, E. S., Andrade, F. K., Brígida, A. I. S., Borges, M. F., Cassales, A. R., Muniz, C. R., Souza Filho, M. de S. M., Morais, J. P. S., Rosa, M. de F.
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Sprache:eng
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Zusammenfassung:ABSTRACT In this study, we have optimized production of bacterial cellulose (BC) by Komagataeibacter hansenii ATCC 23769 in a static cultivation using sisal juice, an agroindustrial residue, as substrate. Optimization of fermentation parameters has been carried out using the one-variable-at-a-time method. Effect of initial sugar concentration, pH, nitrogen supplement, and cultivation time was evaluated. The influence of nitrogen source and quantity for bacterial cellulose production was studied using a central composite rotational design (CCRD).The highest production of BC (3.38 g/L) was obtained after 10 days of cultivation, using sisal juice (pH 5) at 15 g/L of sugars and supplemented with 7.5 g/L of extract yeast. The cellulose production yield in selected sisal culture conditions was three times higher than the yield in synthetic medium, indicating that sisal juice is a suitable substrate for BC production.
ISSN:0104-6632
1678-4383
1678-4383
DOI:10.1590/0104-6632.20170343s20150514