A pseudobiospecific hollow fiber cartridge for in vitro adsorption of autoantibodies from pathological serum
The affinity filtration technique using histidine as a pseudobiospecific ligand immobilized on poly(ethylene vinyl alcohol) hollow fiber membranes (His-PEVA) was used to remove autoantibodies from serum of patients with autoimmune disease. The effects of buffering solution conditions on the efficien...
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Veröffentlicht in: | Brazilian journal of chemical engineering 2000-12, Vol.17 (4-7), p.881-886 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | The affinity filtration technique using histidine as a pseudobiospecific ligand immobilized on poly(ethylene vinyl alcohol) hollow fiber membranes (His-PEVA) was used to remove autoantibodies from serum of patients with autoimmune disease. The effects of buffering solution conditions on the efficiency of autoantibodies removal was studied. The removal of anti-dsDNA, anti-SS-A/Ro, anti-Sm, anti-Sm/RNP and anti-cardiolipin autoantibodies present in the serum was investigated, comparing the efficiency between Hepes and Tris-HCl buffers. The results showed the potential of the membrane to remove all the autoantibodies studied. Anti SS-A/Ro was removed more efficiently in Tris-HCl buffer system rather than with the Hepes buffer. |
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ISSN: | 0104-6632 1678-4383 0104-6632 |
DOI: | 10.1590/S0104-66322000000400052 |