Quantification of Harman Alkaloids in Sour Passion Fruit Pulp and Seeds by a Novel Dual SBSE-LC/Flu (Stir Bar Sorptive Extraction-Liquid Chromatography with Fluorescence Detector) Method

A method for the quantification of the alkaloids harmane and harmine in sour passion fruit (Passiflora edulis f. flavicarpa O. Degener, Passifloraceae) pulp and seeds by stir-bar sorptive extraction and high performance liquid chromatography with fluorescence detection (dual SBSE-LC/Flu) is described. The SBSE parameters were optimized using a fractional factorial design, and the dual SBSE-LC/Flu method was validated following the International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use (ICH) guidelines. The high sensitivity and minimal sample handling of the dual SBSE-LC/Flu method make it attractive for application in phytochemical analysis or in the food industry.