Parent selection for cocoa resistance to witches'-broom

The objective of this work was to identify genotypes with high general combining ability for resistance to witches'-broom (Moniliophthora perniciosa) in populations formed from a first cycle of recurrent selection. Highly productive and resistant clones from different origins were interbred usi...

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Veröffentlicht in:Pesquisa agropecuaria brasileira 2010-07, Vol.45 (7), p.680-685
Hauptverfasser: Silva, Stela Dalva Vieira Midlej, Luz, Edna Dora Martins Newman, Pires, José Luis, Yamada, Milton Macoto, Santos Filho, Lindolfo Pereira dos
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Sprache:eng
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Zusammenfassung:The objective of this work was to identify genotypes with high general combining ability for resistance to witches'-broom (Moniliophthora perniciosa) in populations formed from a first cycle of recurrent selection. Highly productive and resistant clones from different origins were interbred using the North Carolina II design. The clones SCA 6, CSUL 7, RB 39, CEPEC 89, OC 67, BE 4, EEG 29 and ICS 98 were used as paternal parents, while the maternal ones were NA 33, CCN 10, IMC 67, P 4B, CCN 51, CEPEC 86, SGU 54 and ICS 9. Twenty days after germination, 56 seedlings of each cross (four replicates of 14 seedlings) received the inoculation of a 1-mL suspension with 7.5x10(4 ) basidiospores mL-1. Symptoms were evaluated 60 days after inoculation. Significant differences were observed among paternal and among maternal parents, for resistance to witches'-broom assessed according to the proportion of progeny seedlings with the disease symptoms. Differences were also observed between groups of mothers or fathers previously defined as resistant, and groups previously defined as susceptible. It is possible to obtain a combination of genes that can increase the level of resistance to witches'-broom directly from the first cycle of recurrent selection. O objetivo deste trabalho foi identificar genótipos com alta capacidade geral de combinação quanto à resistência à vassoura-de-bruxa (Moniliophthora perniciosa), em populações formadas a partir do primeiro ciclo de seleção recorrente. Clones altamente produtivos e resistentes à vassoura-de-bruxa, de diferentes procedências, foram intercruzados com uso do delineamento Carolina do Norte II. Como progenitores paternos, foram utilizados os clones SCA 6, CSUL 7, RB 39, CEPEC 89, OC 67, BE 4, EEG 29 e ICS 98 e, como maternos, NA 33, CCN 10, IMC 67, P 4B, CCN 51, CEPEC 86, SGU 54 e ICS 9. Vinte dias após a germinação, 56 plântulas de cada cruzamento (quatro repetições de 14 plântulas) receberam inoculação de 1 mL de suspensão com 7,5x10(4) basidiósporos mL-1. Os sintomas foram avaliados 60 dias após a inoculação. Foram observadas diferenças significativas, entre os progenitores paternais e entre os progenitores maternais, quanto à resistência à vassoura-de-bruxa avaliada pela proporção de plântulas das progênies com sintomas da doença. Diferenças também foram observadas entre grupos de progenitores paternais ou maternais previamente definidos como resistentes, e grupos previamente definidos como suscetíveis. É possível obter, d
ISSN:0100-204X
1678-3921
0100-204X
DOI:10.1590/S0100-204X2010000700007