Detection of codon 12 mutation in the K-ras oncogene in pancreatic tumors

Mutations at codons 12, 13, or 61 of the H-ras, K-ras, and N-ras have been detected in human neoplasias by a variety of techniques. Some of these techniques are very sensitive and can detect K-ras mutation in 90% of the cases of pancreatic adenocarcinomas. We analyzed 11 samples of pancreatic adenoc...

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Veröffentlicht in:Revista do Hospital das Clinicás 1999, Vol.54 (1), p.17-20
Hauptverfasser: Kubrusly, M S, Matheucci Júnior, E, Leite, K R, Coelho, A M, Monte, O, Machado, M C, Pinotti, H W
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Sprache:eng
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Zusammenfassung:Mutations at codons 12, 13, or 61 of the H-ras, K-ras, and N-ras have been detected in human neoplasias by a variety of techniques. Some of these techniques are very sensitive and can detect K-ras mutation in 90% of the cases of pancreatic adenocarcinomas. We analyzed 11 samples of pancreatic adenocarcinoma, three samples of pancreatic mucinous cystadenoma, and two samples without tumors in formalin-fixed paraffin embedded tissue sections. K-ras mutations at codon 12 were detected by a two-step PCR-enriched technique in all the samples of pancreatic adenocarcinoma, but not in cystadenoma or control samples. This technique may be useful for early detection of pancreatic cancer.
ISSN:0041-8781
1678-9903
0041-8781
DOI:10.1590/S0041-87811999000100004