Lack of Endotoxin in Borrelia hispanica and Treponema pallidum 1
Abstract Borrelia hispanica from infected guinea pigs and Treponema pallidum from testicular syphilomas of rabbits were assayed for the presence of endotoxin with the Limulus lysate test. A suspension of Borrelia, containing 1.3 × 108 spirochetes/ml, was nonreactive both when it was tested as intact...
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Veröffentlicht in: | Proceedings of the Society for Experimental Biology and Medicine 1983-10, Vol.174 (1), p.47-52 |
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Hauptverfasser: | , |
Format: | Artikel |
Sprache: | eng |
Online-Zugang: | Volltext |
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Zusammenfassung: | Abstract
Borrelia hispanica from infected guinea pigs and Treponema pallidum from testicular syphilomas of rabbits were assayed for the presence of endotoxin with the Limulus lysate test. A suspension of Borrelia, containing 1.3 × 108 spirochetes/ml, was nonreactive both when it was tested as intact organisms, and when tested after disruption of the spirochetes by sonication. Eight different suspensions of treponemes, ranging from 0.6 × 109 to 3 × 109 treponemes/ml, were negative at a 1:10 dilution and were no more active than control suspensions of normal rabbit testes. Therefore, it was concluded that T. pallidum, as well as the Borrelia, possessed no endotoxin. |
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ISSN: | 0037-9727 1535-3699 |
DOI: | 10.3181/00379727-174-41702 |