Para-aminohippurate and Tetraethylammonium Transport in Fragments of Rat Renal Cortex 1

Summary To develop a simple in vitro procedure which, compared to slices, yields less variation in organic anion and cation transport, we used fragments of renal cortex. Each fragment contained 11–15 glomeruli and, after 90 min of incubation, showed normal brush border and well-preserved epithelium. With this technique, there was less variation in transport studies within a given experiment, and fragments responded in the same manner as slices to addition of acetate and serum to the medium.