Studies on an Oncogenic Avian Adenovirus (CELO) I. Biophysical Characterization

Summary CELO virus, from infected allantoic fluids, was treated with 1.0% sodium deoxycholate and 0.01% trypsin and centri-fuged to equilibrium in a rubidium chloride gradient. Two bands, at densities of approximately 1.34 and 1.29 g/cm3, were consistently found. Plaque titrations indicated that the band at 1.34 g/cm3contained the majority of infectious virus. Both hemagglutinating and CF activities were associated with these bands; however, soluble CF antigens were also present in the less dense regions of the gradients. The infectivity of purified CELO virions was heat stable and the virions did not contain the adenovirus group antigen.