Passive Cutaneous Anaphylaxis in the Guinea Pig as Related to the Response of Isolated Ileum

Summary Guinea pigs were sensitized by intravenous injection of known amounts of antibody or antigen and challenged after varying latency periods with the other reactant. A comparison of the results obtained by local accumulation of dye after intradermal challenge (Ovary test) and by the classical Schultz-Dale test showed that the former behaves as a more sensitive test, particularly when small amounts of antibodies or antigen are involved and after inadequate latency periods. It is therefore justifiable to use Ovary's test as a reliable substitute for the Schultz-Dale technic in qualitative and quantitative assays of anaphylactic sensitization.