Amplification-free detection of using an acidic deoxyribozyme-based paper device

We reported a colorimetric paper-based device by integrating the modified acid RNA-cleaving DNAzymes (MaRCD-EC1) for highly sensitive (detection limit = 10 2 CFU mL −1 ), and rapid (within 30 min) detection of E. coli without amplification. This device exhibited a clinical sensitivity of 100% and a specificity of 100% in identifying E. coli -associated urinary tract infections (UTIs) using the clinical urine samples. An acidic deoxyribozyme-coupled amplification-free paper device was constructed to detect Escherichia coli with high sensitivity.