A dual responsive novel bipyridyl carbohydrazide Schiff base as a colorimetric-fluorescent probe for In and Al ions and its potential applications

The development of a probe for the selective recognition of trivalent cations Al 3+ and In 3+ has recently received greater attention due to the crucial roles these metal ions play in many biotic and material processes. In this regard, [2,2′-bipyridine] 6,6′-dicarbohydrazide has been designed to synthesize a Schiff-base ( L ) using diethyl amino salicylaldehyde for the selective detection of Al 3+ and In 3+ . The probe L exhibits selective colorimetric response at 444 nm with In 3+ and turn-on fluorescence response at 497 nm with Al 3+ in DMSO : H 2 O (7 : 3, pH = 7.4). The probe L could form a 1 : 2 [L : M] stoichiometry complex confirmed by Job's plot and ESI mass analysis with an estimated association constant of 9.38 × 10 3 M −2 and 4.75 × 10 4 M −2 with Al 3+ and In 3+ ions, respectively. The probe could detect Al 3+ and In 3+ down to 10.98 nM and 110 nM without much interference from other common interfering ions. Inhibition of C&z.dbd;N isomerization and chelation-enhanced fluorescence (CHEF) could be induced to vary the photophysical properties of L upon interaction with Al 3+ , whereas, the L with In 3+ , exhibits ligand-to-metal charge transfer (LMCT) due to the presence of vacant f-orbitals. The binding mechanism of probe L with metal ions was investigated using 1 H NMR, mass, and DFT studies. More importantly, the potential application of probe L could be used to quantify Al 3+ and In 3+ in water samples and Al 3+ in Dr G cells and a zebrafish in vivo imaging model. The development of a probe for the selective recognition of trivalent cations Al 3+ and In 3+ has recently received greater attention due to the crucial roles these metal ions play in many biotic and material processes.