Enabling a universal lateral flow readout for DNA strand displacement disassembling chemical labels

Herein, we describe a novel strategy that enables lateral flow readout for DNA strand displacement via disassembling chemical labels (DCL). Comparing it to a classic fluorogenic assay, we demonstrate that our DCL-based lateral flow assay is highly sensitive and specific, capable of discriminating single nucleotide variants in buccal swab samples. A novel disassembling chemical label strategy is developed to enable universal lateral flow readout for DNA strand displacement reactions.