Introduction of a multilayered fluorescent nanofilm into lateral flow immunoassay for ultrasensitive detection of in food samples

Fast and sensitive identification of foodborne bacteria in complex samples is the key to the prevention and control of microbial infections. Herein, an ultrasensitive lateral flow assay (LFIA) based on multilayered fluorescent nanofilm (GO/DQD)-guided signal amplification was developed for the rapid and quantitative determination of Salmonella typhimurium ( S. typhi ). The film-like GO/DQD was prepared through the electrostatic mediated layer-by-layer assembly of numerous carboxylated CdSe/ZnS quantum dots (QDs) onto an ultrathin graphene oxide (GO) nanosheet, which possessed advantages including higher QD loading, larger surface areas, superior luminescence, and better stability, than traditional spherical nanomaterials. The antibody-modified GO/DQD can effectively attach onto a target bacterial cell to form a GO/DQD-bacteria immunocomplex containing almost ten thousand QDs, thus greatly improving the detection sensitivity of LFIA. The constructed GO/DQD-LFIA biosensor achieved the rapid and sensitive detection of S. typhi in 14 min with detection limits of as low as 9 cells/mL. Moreover, compared with traditional LFIA techniques for bacteria detection, the proposed assay exhibited excellent stability and accuracy in real food samples and enormously improved sensitivity (2-3 orders of magnitude), demonstrating its great potential in the field of rapid diagnosis. An ultrasensitive LFIA based on multilayered fluorescent nanofilm-guided signal amplification was developed for the rapid and quantitative determination of Salmonella typhimurium in complex food samples.