F-Tagged metal binding pharmacophores for NMR screening of metalloenzymes
This study demonstrates the screening of a collection of twelve 19 F-tagged metal-binding pharmacophores (MBPs) against the Zn( ii )-dependent metalloenzyme human carbonic anhydrase II (hCAII) by 19 F NMR. The isomorphous replacement of Zn( ii ) by Co( ii ) in hCAII produces enhanced sensitivity and...
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| Veröffentlicht in: | Chemical communications (Cambridge, England) England), 2021-05, Vol.57 (4), p.4934-4937 |
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| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Online-Zugang: | Volltext |
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| Zusammenfassung: | This study demonstrates the screening of a collection of twelve
19
F-tagged metal-binding pharmacophores (MBPs) against the Zn(
ii
)-dependent metalloenzyme human carbonic anhydrase II (hCAII) by
19
F NMR. The isomorphous replacement of Zn(
ii
) by Co(
ii
) in hCAII produces enhanced sensitivity and reveals the potential of
19
F NMR-based techniques for metalloenzyme ligand discovery.
This study demonstrates the screening of a collection of twelve
19
F-tagged metal-binding pharmacophores (MBPs) against the Zn(
ii
)-dependent metalloenzyme human carbonic anhydrase II (hCAII) by
19
F NMR. |
|---|---|
| ISSN: | 1359-7345 1364-548X |
| DOI: | 10.1039/d1cc01231b |