A gold-based strip sensor for the detection of benzo[]pyrene in edible oils

This report describes the development of a sensitive and broadly specific indirect competitive enzyme-linked immunosorbent assay ( ic ELISA) and a gold nanoparticle-based immunochromatographic strip (GNP-ICS) assay for the detection of benzo[ a ]pyrene (B[ a ]P), using an anti-B[ a ]P monoclonal antibody (mAb). A broad-specific anti-B[ a ]P mAb (4E8) was raised from two types of haptens, with half maximal inhibitory concentrations and limits of detection (LOD) values of 2.51 and 0.54 ng mL −1 , respectively. In addition, the cross-reactivity was up to 390% with structurally related compounds. The GNP-ICS assay based on a GNP-labeled mAb showed broad specificity in the detection of B[ a ]P and its analogues, with cut-off and visual LOD values of 100 and 10 ng mL −1 , respectively. Furthermore, the recoveries from the developed ic ELISA and GNP-ICS assay in edible oil samples spiked with B[ a ]P were validated by high-performance liquid chromatography-fluorescence detection. The results revealed that the ic ELISA could reliably detect B[ a ]P in edible oils. A broad-specific anti-B[ a ]P mAb (4E8) was prepared with IC 50 value of 2.51 ng mL −1 . The GNP-ICS assay showed broad specificity in the detection of B[ a ]P and its analogues, with cut-off and vLOD values of 100 and 10 ng mL −1 , respectively.