Adsorption of proteins on oral Zn doped iron oxide nanoparticles in mouse stomach and : triggering nanoparticle aggregation

Oral route is one of the most important portals of nanoparticle entry to the body. However, in vivo protein corona formed in the gastrointestinal tract has not been studied owing to the difficulty for the recovery of nanoparticles from the in vivo environment. In this study, by using the magnetic property of iron oxide nanoparticles (Fe 3 O 4 NPs) and Zn 2+ doped iron oxide nanoparticles (Zn 0.4 Fe 2.6 O 4 NPs), the nanoparticles were separated from the gastric fluid after oral administration in mice. The effects of Zn 2+ doping and static magnetic field (SMF) treatment on the protein adsorption on the nanoparticles were investigated in vitro and in vivo . Zn 2+ doping decreases the adsorption of pepsin on the nanoparticles in vitro and affects the composition of the protein corona in vivo and enhances protein adsorption-induced aggregation of the nanoparticles in vitro and in vivo . SMF treatment affects the composition of the protein corona of Fe 3 O 4 NPs and Zn 0.4 Fe 2.6 O 4 NPs, and enhances the aggregation of Fe 3 O 4 NPs and Zn 0.4 Fe 2.6 O 4 NPs in vivo . Furthermore, the results demonstrate that electrostatic attraction is the crucial force to drive adsorption of proteins on Fe 3 O 4 NPs and Zn 0.4 Fe 2.6 O 4 NPs and protein adsorption-induced change in the surface charge of nanoparticles plays an important role in the pH-dependent aggregation of the nanoparticles. In addition, the work provides the evidence that the protein adsorption-induced aggregation of Fe 3 O 4 NPs and Zn 0.4 Fe 2.6 O 4 NPs has no effect on their magnetic susceptibility. The results highlight that Zn 0.4 Fe 2.6 O 4 NPs may be used as a potential oral magnetic resonance imaging contrast agent in diagnosis of gastrointestinal disease. Protein adsorption-induced aggregation of Zn 0.4 Fe 2.6 O 4 nanoparticles had no effect on their magnetic susceptibility.