Advances in targeting and heterologous expression of genes involved in the synthesis of fungal secondary metabolites
The revolutionary discovery of penicillin only marks the start of our exploration for valuable fungal natural products. Advanced genome sequencing technologies have translated the fungal genome into a huge reservoir of "recipes" - biosynthetic gene clusters (BGCs) - for biosynthesis. Study...
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| Veröffentlicht in: | RSC advances 2019-10, Vol.9 (6), p.35124-35134 |
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| container_title | RSC advances |
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| creator | Qiao, Yun-Ming Yu, Rui-Lin Zhu, Ping |
| description | The revolutionary discovery of penicillin only marks the start of our exploration for valuable fungal natural products. Advanced genome sequencing technologies have translated the fungal genome into a huge reservoir of "recipes" - biosynthetic gene clusters (BGCs) - for biosynthesis. Studying complex fungal genetics demands specific gene manipulation strategies. This review summarizes the current progress in efficient gene targeting in fungal cells and heterologous expression systems for expressing fungal BGCs of fungal secondary metabolites.
Gene targeting involves integration of foreign DNA into the fungal genome by several strategies including
Agrobacterium tumefaciens
-mediated transformation (ATMT). |
| doi_str_mv | 10.1039/c9ra06908a |
| format | Article |
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Agrobacterium tumefaciens
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Gene targeting involves integration of foreign DNA into the fungal genome by several strategies including
Agrobacterium tumefaciens
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Gene targeting involves integration of foreign DNA into the fungal genome by several strategies including
Agrobacterium tumefaciens
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| source | DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; PubMed Central Open Access |
| subjects | Biosynthesis Chemistry Fungi Gene expression Gene sequencing Genomes Metabolites Natural products Penicillin |
| title | Advances in targeting and heterologous expression of genes involved in the synthesis of fungal secondary metabolites |
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