Advances in targeting and heterologous expression of genes involved in the synthesis of fungal secondary metabolites

The revolutionary discovery of penicillin only marks the start of our exploration for valuable fungal natural products. Advanced genome sequencing technologies have translated the fungal genome into a huge reservoir of "recipes" - biosynthetic gene clusters (BGCs) - for biosynthesis. Studying complex fungal genetics demands specific gene manipulation strategies. This review summarizes the current progress in efficient gene targeting in fungal cells and heterologous expression systems for expressing fungal BGCs of fungal secondary metabolites. Gene targeting involves integration of foreign DNA into the fungal genome by several strategies including Agrobacterium tumefaciens -mediated transformation (ATMT).