Probing Ca-induced conformational change of calmodulin with gold nanoparticle-decorated single-walled carbon nanotube field-effect transistors

Nanomaterials are ideal for electrochemical biosensors, with their nanoscale dimensions enabling the sensitive probing of biomolecular interactions. In this study, we compare field-effect transistors (FET) comprised of unsorted (un-) and semiconducting-enriched (sc-) single-walled carbon nanotubes (SWCNTs). un-SWCNTs have both metallic and semiconducting SWCNTs in the ensemble, while sc-SWCNTs have a >99.9% purity of semiconducting nanotubes. Both SWCNT FET devices were decorated with gold nanoparticles (AuNPs) and were then employed in investigating the Ca 2+ -induced conformational change of calmodulin (CaM) - a vital process in calcium signal transduction in the human body. Different biosensing behavior was observed from FET characteristics of the two types of SWCNTs, with sc-SWCNT FET devices displaying better sensing performance with a dynamic range from 10 −15 M to 10 −13 M Ca 2+ , and a lower limit of detection at 10 −15 M Ca 2+ . Nanoelectronic detection of ion-induced protein conformational changes with metal nanoparticle-decorated carbon nanotube FET.