The eukaryotic tRNA-guanine transglycosylase enzyme inserts queuine into tRNA a sequential bi-bi mechanism

Eukaryotic tRNA-guanine transglycosylase (TGT) - an enzyme recently recognised to be of potential therapeutic importance - catalyses base-exchange of guanine for queuine at the wobble position of tRNAs associated with 4 amino acids via a distinct mechanism to that reported for its eubacterial homologue. The presence of queuine is unequivocally required as a trigger for reaction between the enzyme and tRNA and exhibits cooperativity not seen using guanine as a substrate. The mechanism of catalysis associated with the human TGT enzyme has been elucidated and differs from that associated with its eubacterial counterpart.