Highly selective and wash-free visualization of resistant bacteria with a relebactam-derived fluorogenic probe

Reported herein is a relebactam-derived fluorogenic reagent for covalent labeling of serine β-lactamases (SBLs), which are the major causes of bacterial resistance to β-lactam antibiotics. This highly selective imaging reagent generates over 300-fold stronger near-infrared fluorescence signals upon...

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Veröffentlicht in:	Chemical communications (Cambridge, England) England), 2019-08, Vol.55 (67), p.9919-9922
Hauptverfasser:	Chen, Yefeng, Xu, Minqiu, Xu, Weipan, Song, Heng, Hu, Liqiang, Xue, Shuyuan, Zhang, Shuangzhan, Qian, Xiana, Xie, Hexin
Format:	Artikel
Sprache:	eng
Schlagworte:	Affinity Labels - chemical synthesis Affinity Labels - chemistry Affinity Labels - pharmacology Amides Antibiotics Azabicyclo Compounds - chemical synthesis Azabicyclo Compounds - chemistry Azabicyclo Compounds - pharmacology Bacteria beta-Lactamase Inhibitors - chemical synthesis beta-Lactamase Inhibitors - chemistry beta-Lactamase Inhibitors - pharmacology beta-Lactamases - chemistry Bonding strength Enterobacter cloacae - enzymology Enterobacter cloacae - isolation & purification Fluoresceins - chemical synthesis Fluoresceins - chemistry Fluoresceins - pharmacology Fluorescence Fluorescent Dyes - chemical synthesis Fluorescent Dyes - chemistry Fluorescent Dyes - pharmacology Indoles - chemical synthesis Indoles - chemistry Indoles - pharmacology Reagents Visualization
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Beschreibung
Zusammenfassung:	Reported herein is a relebactam-derived fluorogenic reagent for covalent labeling of serine β-lactamases (SBLs), which are the major causes of bacterial resistance to β-lactam antibiotics. This highly selective imaging reagent generates over 300-fold stronger near-infrared fluorescence signals upon covalently bonding to SBLs, allowing wash-free visualization of live antimicrobial-resistant bacteria. An unprecedented relebactam-based fluorogenic probe is reported for the wash-free imaging of resistant bacteria.
ISSN:	1359-7345 1364-548X
DOI:	10.1039/c9cc04533c