Internalization of the pseudorabies virus macropinocytosis analyzed by quantum dot-based single-virus tracking

With the aid of the single-virus tracking technique and the quantum dot-labeling strategy, the internalization of the pseudorabies virus (PrV) has been visualized in real time. The results demonstrate that macropinocytosis can be considered as a major pathway for PrV entering HeLa cells, which facilitates the development of therapeutics for virus-triggered diseases. Utilizing a single-virus tracking technique, the key steps of the PrV entry process were analyzed in detail by real-time monitoring of quantum dot-labeled PrV.