Energy landscape of polymorphic amyloid generation of β2-microglobulin revealed by calorimetryElectronic supplementary information (ESI) available. See DOI: 10.1039/c8cc02718h

Understanding of amyloid aggregation in terms of thermodynamics and kinetics is still limited. We herein examined the mechanism of β2-microglobulin amyloidogenesis using our unique method of isothermal titration calorimetry-based thermodynamic/kinetic measurements, and revealed the energy landscape of polymorphic amyloidogenesis under biological environment-mimicking conditions including shear forces and crowding effects. ITC-based energy landscape provides a comprehensive understanding of amyloid aggregation in terms of thermodynamics and kinetics.