Designing the thermal behaviour of aqueous biphasic systems composed of ammonium-based zwitterionsElectronic supplementary information (ESI) available: Materials and experimental procedure, synthesis and purity of ammonium based zwitterions, binodal weight fraction data and phase diagrams representation. See DOI: 10.1039/c7gc02262j

The ability of water-soluble ammonium-based zwitterions (ZIs) to form aqueous biphasic systems (ABS) in the presence of aqueous solutions of salts is disclosed here. These systems are thermoreversible at temperatures close to room temperature and further allow the design of their thermal behavior, from an upper critical solution temperature (UCST) to a lower critical solution temperature (LCST), by increasing the ZI alkyl chain length. The investigated thermoreversible ABS are more versatile than typical liquid-liquid systems, and can be applied in a wide range of temperatures and compositions envisaging a target separation process. An example is given regarding their ability to selectively separate mixtures of aromatic and aliphatic amino acids in a single-step. The design of the thermal behaviour - from an upper critical solution temperature (UCST) to a lower critical solution temperature (LCST) - of aqueous biphasic systems composed of water-soluble ammonium-based zwitterions.