Iron chelators inhibit the heme-degradation reaction by HutZ from Vibrio choleraeElectronic supplementary information (ESI) available: Materials and methods, and supplementary figures. See DOI: 10.1039/c7dt00121e

HutZ is a heme-degrading enzyme. We found that the heme-degradation reaction by HutZ is inhibited by the iron chelators. Kinetic analysis of each heme-degradation step suggests that water molecules hydrogen bonded to Thr27 are involved in proton transfer to Fe( iii )-OO − , and that this step is inhibited by iron chelators. The heme-degradation reaction by HutZ from Vibrio cholerae is inhibited by the iron chelators.