SpyCatcher-SpyTag mediated labelling of progeny baculovirus with quantum dots for tracking viral infection in living cells

A non-invasive labelling strategy is proposed to label baculovirus via genetic insertion of a SpyTag into the viral glycoprotein, followed by specific conjugation with the SpyCatcher protein on modified quantum dots (QDs) through an isopeptide bond. The labelling method is convenient and efficient a...

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2018-01, Vol.54 (1), p.1189-1192
Hauptverfasser: Ke, Xianliang, Zhang, Yuan, Zheng, Fangliang, Liu, Yan, Zheng, Zhenhua, Xu, Yi, Wang, Hanzhong
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Zusammenfassung:A non-invasive labelling strategy is proposed to label baculovirus via genetic insertion of a SpyTag into the viral glycoprotein, followed by specific conjugation with the SpyCatcher protein on modified quantum dots (QDs) through an isopeptide bond. The labelling method is convenient and efficient and shows little attenuation of viral infectivity. Therefore, it is a biologically compatible technique for tracking viral infection. A non-invasive labelling strategy is proposed to label baculovirus via genetic insertion of a SpyTag into the viral glycoprotein, followed by specific conjugation with the SpyCatcher protein on modified quantum dots (QDs) through an isopeptide bond.
ISSN:1359-7345
1364-548X
DOI:10.1039/c7cc08880a