Programming membrane permeability using integrated membrane pores and blockers as molecular regulators

We report a bottom-up synthetic biology approach to engineering vesicles with programmable permeabilities. Exploiting the concentration-dependent relationship between constitutively active pores (alpha-hemolysin) and blockers allows blockers to behave as molecular regulators for tuning permeability, enabling us to systematically modulate cargo release kinetics without changing the lipid fabric of the system. Vesicle permeability could be tuned using α-hemolysin pores and varying concentrations of TRIMEB blockers, which act as molecular regulators