Quaternized -chloramine coated magnetic nanoparticles: a trifecta of superior antibacterial activity, minimal residual toxicity and rapid site removal

We propose a new and highly effective tool to add to the ever shrinking toolbox for combating infections caused by antibiotic resistant bacteria; N -chloramine and quaternized N -chloramine were coated onto iron-oxide magnetic nanoparticles to generate antibacterial MNPs. Two differently-sized primary iron-oxide nanoparticles (3 nm and 10 nm) were synthesized and coated with silica and (3-chloropropyl)triethoxysilane, allowing subsequent introduction of N -chloramine precursors - dimethyl hydantoin (DMH) and quaternized dimethyl hydantoin (QDMH). The functionalized MNPs (MNP@DMH and MNP@QDMH) have a clear core-shell structure as evidenced by TEM images. Fe 3 O 4 was identified (by combining X-ray diffraction with Mössbauer spectroscopy) to be the iron oxide in the 10 nm MNPs, while γ-Fe 2 O 3 and Fe 3 O 4 were the 3 nm MNP's oxide phases. Both MNPs (3 nm and 10 nm) have good magnetic responses, with saturation magnetizations of 40 ± 4 emu g −1 and 65 ± 2 emu g −1 , respectively. Chlorination activated the antibacterial function and yielded two antibacterial MNPs: MNP@DMCl and MNP@QASCl. At the equivalent [Cl + ] of 50 ppm, both coatings demonstrated fast inactivation of the model bacteria methicilin-resistant Staphylococcus aureus (MRSA) and multi-drug resistant (MDR) Pseudomonas aeruginosa . For either size of primary MNPs, MNP@QDMCl is more effective than MNP@DMCl. A hand-held magnet could quickly remove >99% of the functionalized MNPs from a wound simulant within 2 minutes. Applying nanoscience and nanotechnology to potentially allow the safe use of potent broad-spectrum biocides on topical wounds.