Synthesis of triazole-linked morpholino oligonucleotides Cu catalysed cycloaddition

Triazole-linked morpholino ( TL MO) oligonucleic acids were synthesised using the Cu I catalysed (3 + 2) azide-alkyne cycloaddition (CuAAC) reaction. The modified DNA analogues were incorporated into 13-mer sequences via solid phase synthesis. UV melting experiments showed that the TL MO modification gives higher T m values than the corresponding TL DNA modification. Triazole-linked morpholino ( TL MO) oligonucleic acids were synthesised using the Cu I catalysed (3 + 2) azide-alkyne cycloaddition (CuAAC) reaction.