Sprayable peptide-modified silver nanoparticles as a barrier against bacterial colonizationElectronic supplementary information (ESI) available: Experimental details and supplementary figures. See DOI: 10.1039/c6nr07976h

Sprayable formulations of AgNPs were prepared by exchanging citrate capping agents with LL37-SH peptides. The AgNP@LL37 material was then combined with type I collagen to form a stable film once sprayed. The AgNP@LL37 spray prevented Pseudomonas aeruginosa ( P. aeruginosa ) proliferation and eradicated a P. aeruginosa biofilm, while being non-toxic for human skin fibroblasts embedded within 3D artificial skin constructs. Finally, no silver infiltration was observed after spraying on a full-thickness skin wound in a mouse model. Nanosilver spray-on formulation provides antimicrobial and antibiofilm protection with non-measurable toxicity for skin cells and no in vivo organ infiltration.