Interference-free analysis of aflatoxin B and G in various foodstuffs using trilinear component modeling of excitation-emission matrix fluorescence data enhanced through photochemical derivatization
A novel 'dilute-and-shoot' analytical strategy coupling a self-weighted alternating normalized residue fitting (SWANRF) algorithm with two-dimensional fluorescence detection enhanced through photochemical derivatization (PD) was proposed in the present work for rapid, simultaneous and accu...
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Veröffentlicht in: | RSC advances 2016-03, Vol.6 (31), p.2585-25863 |
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Zusammenfassung: | A novel 'dilute-and-shoot' analytical strategy coupling a self-weighted alternating normalized residue fitting (SWANRF) algorithm with two-dimensional fluorescence detection enhanced through photochemical derivatization (PD) was proposed in the present work for rapid, simultaneous and accurate quantitative analysis of aflatoxin B
1
and G
1
in various foodstuffs (including cereals, honey, and edible oil). By coupling the predominant second-order advantage of the SWANRF algorithm with the ultra-sensitivity of fluorescence detection enhanced through off-line photochemical derivatization, the specific quantitative information of both analytes could be successfully extracted from heavily interferential matrices without complicated multi-step purification and chromatographic separation procedures. Consequently, the whole analytical time and expense were significantly decreased, accurate recoveries (with relative standard deviations, RSDs) (93.5 ± 6.6-102.8 ± 4.0% for AFB
1
, and 96.4 ± 3.6-107.2 ± 6.0% for AFG
1
) and extremely low limits of detection (LODs) (0.12-0.21 ng mL
−1
for AFB
1
, and 0.27-0.75 ng mL
−1
for AFG
1
) were obtained for analytical foodstuff matrices. In addition, all quantitative results of this proposed strategy were carefully compared with the standard IAC-LC-ESI
+
-MS method for further confirmation, which proved that SWANRF-EEMs are promising as an alternative analytical strategy for the routine analysis of multiplex aflatoxins, and a theoretical basis for developing portable detecting devices.
A novel 'dilute-and-shoot' strategy coupling self-weighted alternating normalized residue fitting algorithm with two-dimensional fluorescence detection enhanced by photochemical derivatization was proposed for rapid analysis of aflatoxin B
1
and G
1
. |
---|---|
ISSN: | 2046-2069 |
DOI: | 10.1039/c5ra26549e |