Self-assembly, doxorubicin-loading and antibacterial activity of well-defined ABA-type amphiphilic poly(N-vinylpyrrolidone)-b-poly(d,l-lactide)-b-poly(N-vinyl pyrrolidone) triblock copolymers

A series of ABA type well-defined amphiphilic poly( N -vinylpyrrolidone) (PNVP)- b -poly( d , l -lactide)- b -PNVP triblock copolymers have been synthesized via the combination of ring opening polymerization and xanthate-mediated reversible addition-fragmentation chain transfer polymerization, and analyzed by 1 H NMR spectroscopy and gel permeation chromatography. Aggregation properties of these amphiphilic triblock copolymers have been revealed by fluorescence spectroscopy, transmission electron microscopy and dynamic light scattering, and supported by 1 H NMR spectroscopy. Doxorubicin (DOX) has successfully been loaded into the block copolymer micelles with a loading efficiency of 37.5%. DOX-loaded PNVP 51 - b -PDLLA 48 - b -PNVP 51 block copolymer showed sustained release within 36 h. Antibacterial properties of DOX-loaded micelles have been found to be significantly effective with respect to free DOX in terms of minimum inhibitory concentration, disk diffusion assay, growth curve, bacterial reduction and enzymatic assay based on in vitro studies. Synthesis, self-assembly, DOX-loading and antibacterial activity of well-defined ABA-type amphiphilic poly( N -vinylpyrrolidone)- b -poly( d , l -lactide)- b -poly( N -vinylpyrrolidone) triblock copolymers.