Click and release: fluoride cleavable linker for mild bioorthogonal separation

Herein, we present a water dispersable, magnetic nanoparticle supported "click and release" system. The cleavable linker has been synthesized by using a strain-promoted copper-free "click" reagent to establish the specific link and a fluoride cleavable silane moiety for mild cleavage. Small organic molecules, azide-bearing dyes and functionalized enzymes have been bound to the magnetic particle and released in a bioorthogonal way. A water dispersable, magnetic nanoparticle supported, bioorthogonal "click and release" system has been synthesized.