Assembly of bioactive multilayered nanocoatings on pancreatic islet cells: incorporation of α1-antitrypsin into the coatingsElectronic supplementary information (ESI) available: Experimental method, Fig. S1-S4. See DOI: 10.1039/c5cc02570b

A spontaneous multilayer deposition approach for presenting therapeutic proteins onto pancreatic islet surfaces, using a heparin polyaldehyde and glycol chitosan alternating layering scheme, has been developed to enable the nanoscale engineering of a microenvironment for transplanted cells. The nanocoating incorporating α1-antitrypsin, an anti-inflammatory protein, exhibited effective anti-coagulant activities in vitro . A novel multilayer deposition approach to the delivery of therapeutic proteins onto the surface of pancreatic islets, using a heparin polyaldehyde and glycol chitosan alternating layering scheme, has been developed for addressing the blood-mediated inflammatory reaction against islet cells.