Selective inhibition of the hypoxia-inducible factor prolyl hydroxylase PHD3 by Zn(ii)Electronic supplementary information (ESI) available: Experimental details. See DOI: 10.1039/c5cc02143j

We report herein that Zn( ii ) selectively inhibits the hypoxia-inducible factor prolyl hydroxylase PHD3 over PHD2, and does not compete with Fe( ii ). Independent of the oligomer formation induced by Zn( ii ), inhibition of the activity of PHD3 by Zn( ii ) involves Cys42 and Cys52 residues distantly located from the active site. Zn( ii ) selectively inhibits PHD3 over PHD2 by interacting with Cys42 and Cys52 residues located distantly from the active site.