Outstanding effects on antithrombin activity of modified TBA diastereomers containing an optically pure acyclic nucleotide analogueElectronic supplementary information (ESI) available: Fig. S1-S3 and Tables S1 and S2. See DOI: 10.1039/c4ob00149d

Herein, we report optically pure modified acyclic nucleosides as ideal probes for aptamer modification. These new monomers offer unique advantages in exploring the role played in thrombin inhibition by a single residue modification at key positions of the TBA structure. Optically pure modified acyclic nucleosides offer unique advantages in exploring the effect on thrombin inhibition of single residue modifications at key positions of TBA.