Microarray immobilization of biomolecules using a fast trans-cyclooctene (TCO)-tetrazine reactionElectronic supplementary information (ESI) available. See DOI: 10.1039/c4cc03838j

By anchoring 1,2,4,5-tetrazine-containing biomolecules onto trans -cyclooctene (TCO)-functionalized slides, a site-specific microarray immobilization approach is described in this study. Compared with existing immobilization methods, our approach offers several distinctive features, including fast kinetics and high chemoselectivity. A site-specific microarray immobilization method based on trans -cyclooctene-tetrazine ligation has been developed and applied to anchor a variety of biomolecules.