Quantification of DNA through a fluorescence biosensor based on click chemistry

A simple, sensitive and selective fluorescence biosensor for determination of DNA using CuS particles based on click chemistry is reported. Biotin-modified capture DNA was modified on Streptavidin MagneSphere Paramagnetic Particles (PMPs) and hybridized with target DNA (hepatitis B virus DNA had bee...

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Veröffentlicht in:Analyst (London) 2014-11, Vol.139 (22), p.5669-5673
Hauptverfasser: Yue, Guiyin, Ye, Huazhen, Huang, Xijing, Ye, Wenmei, Qiu, Suyan, Qiu, Bin, Lin, Zhenyu, Chen, Guonan
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Sprache:eng
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Zusammenfassung:A simple, sensitive and selective fluorescence biosensor for determination of DNA using CuS particles based on click chemistry is reported. Biotin-modified capture DNA was modified on Streptavidin MagneSphere Paramagnetic Particles (PMPs) and hybridized with target DNA (hepatitis B virus DNA had been chosen as an example), then bound target DNA was hybridized with DNA-CuS particles and formed a sandwich like structure. CuS particles on the sandwich structures can be destroyed by acid to form Cu( ii ), and Cu( ii ) can be reduced to Cu( i ) by sodium ascorbate, which in turn catalyzes the reaction between a weak-fluorescent 3-azido-7-hydroxycoumarin and propargyl alcohol to form a fluorescent 1,2,3-triazole compound. Using this method, target DNA concentration can be determined by a change in the fluorescence intensity of the system. It is found that the fluorescence increase factor has a direct linear relationship to the logarithm of target DNA concentrations in the range of 0.1 to 100 nM, and the detection limit is 0.04 nM ( S / N = 3). The proposed sensor not only allows high sensitivity and good reproducibility, but also has a good selectivity to single-nucleotide mismatches. A simple, sensitive and selective fluorescence biosensor for determination of DNA using CuS particles based on click chemistry is reported.
ISSN:0003-2654
1364-5528
DOI:10.1039/c4an01438c