Evaluating the sensitivity of hybridization-based epigenotyping using a methyl binding domain proteinElectronic supplementary information (ESI) available. See DOI: 10.1039/c4an00667d

Hypermethylation of CpG islands in gene promoter regions has been shown to be a predictive biomarker for certain diseases. Most current methods for methylation profiling are not well-suited for clinical analysis. Here, we report the development of an inexpensive device and an epigenotyping assay with a format conducive to multiplexed analysis. We explore and quantify an approach for routine DNA hypermethylation analysis that does not require sodium bisulfite treatment.