Assessing the guest-accessible volume in MOFs using two-photon fluorescence microscopy

The assessment of guest-accessible volume in MOFs can be reliably done by using two-photon confocal fluorescence microscopy (TPM) with a tool-box of dyes with a wide range of sizes. It would be applicable to any porous materials, whose single-crystal structures are not available, or non-crystalline materials. For determining the guest-accessible volume of porous materials by eye, especially for MOFs in this case, two-photon fluorescence microscopy with various dyes was successfully employed. It would be applicable to any porous materials, whose single-crystal structure is not available, or non-crystalline materials.