Quantitative detection of single base mutation by combining PNA hybridization and MALDI-TOF mass analysisElectronic supplementary information (ESI) available: Experimental details. See DOI: 10.1039/c3cc00070b

Peptide nucleic acid (PNA) probes were designed to bind to the internal reference sequence and the single base mutation sequence within PCR-amplified DNA templates. PNAs hybridized to the target sequences on DNA were analyzed using MALDI-TOF mass spectrometry. Accurate quantification of the relative amount of mutant DNA was reproducibly demonstrated. Peptide nucleic acid (PNA) probes were designed to bind to the internal reference sequence and the single base mutation sequence within PCR-amplified DNA templates, and the PNA probes were quantitatively analyzed with MALDI-TOF mass spectrometry.