Oxo-ester mediated native chemical ligation on microarrays: an efficient and chemoselective coupling methodologyElectronic supplementary information (ESI) available. See DOI: 10.1039/c2cc30844d

We report a highly efficient and selective method for the coupling of peptides and glycoconjugates bearing N -terminal cysteines to activated surfaces. This chemoselective method generates stable amide linkages without using any thiol additives. A chemoselective method for the coupling of amino acids, peptides and glycoconjugates to solid support is described. The reaction is based on Native Chemical Ligation using oxo-esters without the need for additional thiols.