Application of photoactive yellow protein as a photoresponsive module for controlling hemolytic activity of staphylococcal α-hemolysinElectronic supplementary information (ESI) available: Preparation of mutants, and hemolytic activities of wild-type Hla and N-PYP-Hla-SS. See DOI: 10.1039/c2cc18118e

A chimeric protein ( N -PYP-Hla), consisting of staphylococcal pore-forming toxin α-hemolysin (Hla) and photoactive yellow protein (PYP), exhibited photoresponsive hemolytic activities, where visible light irradiation gave rise to retardation of hemolysis at 25 °C. A chimeric protein, consisting of staphylococcal pore-forming toxin α-hemolysin and photoactive yellow protein, exhibited photoresponsive hemolytic activities, where visible light irradiation gave rise to retardation of hemolysis at 25 °C.