One-step patterning of hollow microstructures in paper by laser cutting to create microfluidic analytical devicesElectronic supplementary information (ESI) available. See DOI: 10.1039/c2an36219h

In this paper, we report a simple, low-cost method for rapid, highly reproductive fabrication of paper-based microfluidics by using a commercially available, minitype CO 2 laser cutting/engraving machine. This method involves only one operation of cutting a piece of paper by laser according to a predesigned pattern. The hollow microstructures formed in the paper are used as the 'hydrophobic barriers' to define the hydrophilic flowing paths. A typical paper device on a 4 cm × 4 cm piece of paper can be fabricated within ∼7-20 s; it is ready for use once the cutting process is finished. The main fabrication parameters such as the applied current and cutting rate of the laser were optimized. The fabrication resolution and multiplexed analytical capability of the hollow microstructure-patterned paper were also characterized. This paper describes a simple laser cutting method for one-step patterning of hollow microstructures in paper to fabricate microfluidic analytical devices that are ready for use once the cutting process is finished.