Fluorescent probe for highly selective and sensitive detection of hydrogen sulfide in living cells and cardiac tissuesElectronic supplementary information (ESI) available: The supporting information provides the detailed synthesis, characterization (NMR, MS, IR etc.) of the probes. H2S selectivity of probe C-6Az, HOMO and LUMO orbits of C-7Az and C-6Az, time dependence of linear relationship between triggered fluorescence and the concentration of H2S, the photostability of C-7Az, detailed experi

A coumarin-based fluorescence chemoprobe was developed and evaluated for the selective and sensitive detection of hydrogen sulfide in degassed PBS buffers and fetal bovine serum. Fluorescence detection of hydrogen sulfide in living cells was also successfully achieved using two-photon confocal fluorescence imaging. Further in situ visualization of endogenous H 2 S was realized in cardiac tissues of normal rats and atherosclerosis (AS) rats. H 2 S visualization is successfully achieved in cells and cardiac tissues with a coumarin-based fluorescence probe.